JOURNAL

Contents

Original Papers

Effectiveness of genotype-based selection in the production of marker-free and genetically fixed transgenic lineages: ectopic expression of a pistil chitinase gene increases leaf-chitinase activity in transgenic rice plants without hygromycin-resistance gene

Fujio Hashizume, Tetsuya Nakazaki, Tohru Tsuchiya, Tsukasa Matsuda. . . . . . . 349 [PDF]

Identification of a 23 kDa protein (P23k) related to the sugar supply in germinating barley seeds

Shin-ichiro Kidou, Ai Oikawa, Naoko Sasaki, Hiroshi Yasuda, Tetsuro Yamashita, Hiroyuki Koiwa, Kiyoaki Kato, Shin-ichiro Ejiri. . . . . . . 357 [PDF]

Characterization of the promoter of the Wiv-1 (Lin6) gene encoding a wound-inducible cell wall-bound acid invertase in tomato

Akio Ohyama, Keita Suwabe, Tsukasa Nunome, Hiroyuki Fukuoka. . . . . . . 365 [PDF]

Biochemical characterization and mutational studies of a chalcone synthase from yellow snapdragon (Antirrhinum majus) flowers

Masayoshi Hatayama, Eiichiro Ono, Keiko Yonekura-Sakakibara, Yoshikazu Tanaka, Tokuzo Nishino, Toru Nakayama. . . . . . . 373 [PDF]

Differential expression of anthocyanin biosynthesis genes in suspension culture cells of Rosa hybrida cv. Charleston

Chandani Kumari Hennayake, Shunsuke Takagi, Kiyoko Nishimura, Michio Kanechi, Yuichi Uno, Noboru Inagaki. . . . . . . 379 [PDF]

Efficacy of a special screened greenhouse covered by duplex fine mesh in reducing maize outcrossing

Shin Watanabe, Tsunenori Sano, Hiroshi Kamada, Hiroshi Ezura. . . . . . . 387 [PDF]

Short Communications

The phenylalanine pathway is the main route of salicylic acid biosynthesis in Tobacco mosaic virus -infected tobacco leaves

Daisuke Ogawa, Nobuyoshi Nakajima, Shigemi Seo, Ichiro Mitsuhara, Hiroshi Kamada, Yuko Ohashi. . . . . . . 395 [PDF]

Immunological detection and cellular localization of the phenylalanine ammonia-lyase of a hybrid aspen

Yuriko Osakabe, Kazuya Nanto, Hiroko Kitamura, Keishi Osakabe, Shinya Kawai, Noriyuki Morohoshi, Yoshihiro Katayama. . . . . . . 399 [PDF]

Increase in peroxidase activity in tea callus in response to darkness, 2,4-dichlorophenoxyacetic acid and carbohydrates

Yoichi Aoshima, Masumi Takemoto. . . . . . . 405 [PDF]

Notes

Technical Note

In vitro plant regeneration from the petioles of primary leaves of mungbean Vigna radiata L.

L. Sita Mahalakshmi, T. Leela, B. Kiran Kumar, B. Naresh, Prathibha Devi. . . . . . . 409 [PDF]

An efficient transient gene expression system using aleurones of diploid wheat seeds

Shigeko Utsugi, Masahiko Maekawa, Kazuhiko Noda. . . . . . . 413 [PDF]

Gene Note

Cloning and molecular analysis of radish (Raphanus sativus L.) cDNAs encoding heterodimeric γ-glutamyltransferases

Yoshihiro Nakano, Rafael Prieto, Masakazu Hara, Jiro Sekiya. . . . . . . 419 [PDF]

Identification of polyamine oxidase genes from apple and expression analysis during fruit development and cell growth

Hiroyasu Kitashiba, Chikako Honda, Takaya Moriguchi. . . . . . . 425 [PDF]

Tissue Culture Note

The combined effect of photoperiod, light intensity and GA3 on adventitious shoot regeneration from cotyledons of spinach (Spinacia oleracea L.)

Sudarshanee Geekiyanage, Tomoyuki Takase, Shin Watanabe, Seiichi Fukai, Tomohiro Kiyosue. . . . . . . 431 [PDF]