Original Papers

Mitochondria use actin filaments as rails for fast translocation in Arabidopsis and tobacco cells

Yoko Doniwa, Shin-ichi Arimura , Nobuhiro Tsutsumi. . . . . . . 441 [PDF]

Fluorescently labeled proteins in transgenic Arabidopsis thaliana showed that mitochondria and peroxisomes moved at similar velocities. Movement of mitochondria was stopped by an actin-depolymerizing drug but not by a microtubule-depolymerizing drug. In living Arabidopsis cells and elongated cultured tobacco cells, mitochondria move along actin filaments as rails. The movement was not related to arrays of microtubules.

Imaging of plant dynamin-related proteins and clathrin around the plasma membrane by variable incidence angle fluorescence microscopy

Masaru Fujimoto, Shin-ichi Arimura, Mikio Nakazono, Nobuhiro Tsutsumi. . . . . . . 449 [PDF] [Supplement 1(avi)] [Supplement 2(avi)] [Supplement 3(avi)]

In variable incidence angle fluorescence microscopy, GFP-labeled Arabidopsis dynamin-related proteins (DRP1A and DRP2B), and clathrin light chain were observed as 200-500nm dot-like puncta around the plasma membrane in tobacco suspension cells. Moreover, these puncta moved to and away from the cell surface or also assembled and disassembled. These results raise the possibility that these three proteins are involved in membrane trafficking around the plasma membrane, including endocytosis.

Isolation of novel gain- and loss-of-function alleles of the circadian clock gene LATE ELONGATED HYPOCOTYL (LHY) in Arabidopsis

Atsushi Oda, Paul H Reeves, Takeomi Tajima, Mayu Nakagawa, Hiroshi Kamada, George Coupland ,Tsuyoshi Mizoguchi. . . . . . . 457 [PDF]

We isolated a novel deletion allele ( lhy-14 ) and a gain-of-function allele ( lhy-2 ) of lhy. lhy-2 was isolated as an intragenic suppressor of lhy-12, one of the loss-of-function alleles of lhy. We propose a model explaining the abnormal splicing of LHY lhy-12 and lhy-2.

Expression analysis of gene trap lines and mapping of donor loci for Dissociation transposition in Arabidopsis

Tomohiko Kato, Satoshi Tabata, Shusei Sato. . . . . . . 467 [PDF]

We constructed a new T-DNA vector carrying the GUS reporter gene for gene trap analysis and the Ds element for targeted mutagenesis, and subsequently produced 51,000 gene trap lines. We analyzed the expression patterns of the GUS reporter gene in 20,000 gene trap lines. One hundred and forty integration sites of the T-DNAs in the gene trap lines were mapped on the genome. The T-DNA contains the Ds element therefore these lines can be used as donor loci for transposition.

Expression patterns of Arabidopsis ERF VIII-b subgroup genes during in vitro shoot regeneration and effects of their overexpression on shoot regeneration efficiency

Hiromi Mase, Miki Hashiba, Naoki Matsuo, Hiroharu Banno. . . . . . . 481 [PDF]

Transcript levels of six ERF VIII-b genes including ESR1 and ESR2 transiently increased 1-2 days after induction by incubation on shoot-inducing medium, although only ESR1 and ESR2 enhanced shoot regeneration by their overexpression.

Calcium-dependent protein phosphorylation in hairy roots of Daucus carota

Ryoichi Kato, Kaori Okuyama, Masahiro Kodama, Shoko Sato, Hiroshi Kamada ,Takashi Suzuki. . . . . . . 487 [PDF]

Three proteins, with molecular masses of 65, 57.5, and 52 kDa, were phosphorylated by calcium-dependent protein kinase, which might be connected with the growth of carrot hairy roots, that are materials having a vigorous elongation.

Anthocyanin acyltransferase engineered for the synthesis of a novel polyacylated anthocyanin

Hirokazu Suzuki, Tokuzo Nishino, Toru Nakayama. . . . . . . 495 [PDF]

A rationally-designed mutant of a malonyl-CoA:anthocyanin 5-O-glucoside-6”’-O-malonyltransferase of Salvia splendens (Ss5MaT1), Ss5AT306, has an acquired specificity for hydroxycinnamoyl-CoA and is able to produce novel polyacylated anthocyanins. One of such products, cyanidin 3-O-(6”-O-p-coumaroylglucoside)-5-O-(6”’-O-p-coumaroylglucoside), was spectrally characterized.

Short Communication

Characterization of three halide methyltransferases in Arabidopsis thaliana

Yukari Nagatoshi, Tatsuo Nakamura. . . . . . . 503 [PDF]

Biochemical analyses of AtHOL1, which is involved in methyl halide emissions in Arabidopsis, and its isoforms designated AtHOL2 and AtHOL3 showed that the three recombinant AtHOL proteins had distinct levels of the S-adenosyl-L-methionine-dependent methyltransferase activities for iodide.


Transgenic Notes

Agrobacterium-mediated transformation of indica rice under Acetosyringone-free conditions

Mangu Venkata Ramana Rao, Gundimeda Jwala Narasimha Rao. . . . . . . 507 [PDF]

The influence of acetosyringone at different steps of Agrobacterium-mediated transformation of rice was assessed. Fertile transgenic plants from indica rice were developed in the absence of acetosyringone suggesting that phenolics like acetosyringone may not be essential for induction of vir genes of Agrobacterium.

Gene Notes

Cloning of cDNAs for DELLA proteins suppressing cysteine proteinase genes in germinated cotyledons of common bean seeds

Daisuke Yamauchi, Masahiro Kobayashi, Tomonori Nakai, Kou Kubota. . . . . . . 513 [PDF]

Two cDNAs encoding DELLA proteins (PvGAI1 and PvGAI2) were isolated from common bean seedlings. We suggest that PvGAI2 is mainly responsible for regulating the expression of proteinase genes in germinated cotyledons.

Identification and genetic relationship among Polyscias and Schefflera (Araliaceae) using RAPD and ISSR markers

Gyana Ranjan Rout , Joyti Kullu, Sunil Kumar Senapati, Subhashree Aparajita, Anuradha Mohapatra. . . . . . . 519 [PDF]

The present investigation was undertaken to study the genetic variation within the species/varieties of Polyscias and Schefflera through RAPD and ISSR markers. A total of 164 and 69 distinct DNA fragments ranging from 300 to 2500 bp were amplified by using selected random RAPD and ISSR primers respectively. The correlation matrix indicate that there was significant correlation between ISSR and RAPD markers. This study will helpful for breeding programs as well as a major input into conservation biology of foliage crop.

Tissue Culture Note

Micropropagation and in vitro flowering in Pentanema indicum Ling

Iyyakkannu Sivanesan, Byoung Ryong Jeong. . . . . . . 527 [PDF]

The frequency of shoot regeneration from callus, node and shoot tip explants were higher on medium containing combinations of auxin and cytokinin than on medium containing cytokinin alone. Flowering can be induced in vitro in excised shoot cultures of Pentanema indicum. Adenine sulfate which has been found to be effective for shoot bud formation in P. indicum also supported the development of floral buds.

Transgenic Note

Production of transgenic plants and their early seed set in Japanese soybean variety, Kariyutaka

Hiroshi Sato, Tetsuya Yamada, Yohichi Kita, Masao Ishimoto, Keisuke Kitamura. . . . . . . 533 [PDF]

Agrobacterium-mediated transformation was performed in Japanese soybean variety, Kariyutaka. Because transgenic shoots of Kariyutaka formed in vitro precocious flower buds, T1 seeds were obtained in only six monthes after co-cultivation with Agrobacterium. These facts indicate that this transformation system can be used for the rapid development of transgenic soybean lines.