Original Papers

Analysis of global changes in gene expression during activity-dormancy cycle in hybrid aspen apex

Anna Karlberg, Madeleine Englund, Anna Petterle, Gergely Molnar, Andreas Sjodin, Laszlo Bako, Rishikesh P. Bhalerao. . . . . . . 1 [PDF] [Supplement 1] [Supplement 2] [Supplement 3] [Supplement 4] [Supplement 5] [Supplement 6] [Supplement 7]

Short day signal induces growth cessation and dormancy in perennial plants e. g. trees of boreal forest. Full genome microarray has been used to elucidate the transcriptional networks underlying the distinct stages of activity-dormancy cycle in apex of hybrid aspen plants.

Metabolic profiling analysis of genetically modified rice seedlings that overproduce tryptophan reveals the occurrence of its inter-tissue translocation

Fumio Matsuda, Atsushi Ishihara, Kojiro Takanashi, Keiko Morino, Haruna Miyazawa, Kyo Wakasa, Hisashi Miyagawa. . . . . . . 17 [PDF] [Supplement]

Transgenic rice plants that over-express the OASA1D gene accumulated Trp at a high concentration without marked effects on the amounts of other major metabolites. Distribution of Trp in the plant was uneven, because of its translocation from old to young leaves.

Cre-loxP mediated marker elimination and gene reactivation at the waxy locus created in rice genome based on strong positive-negative selection

Rie Terada, Miki Nagahara, Kazuhiko Furukawa, Miki Shimamoto, Katsushi Yamaguchi, Shigeru Iida. . . . . . . 29 [PDF]

The targeted-waxy was generated by the insertion of a positive marker, which was between a pair of loxP, to the Waxy locus by a strong positive-negative selection-mediated rice gene targeting. We report the detection of Waxy reactivation after the exposition of the targeted-waxy locus to the transiently expressed Cre.

Isolation of multidrug resistance associated protein like gene from lead hyperaccumulator common buckwheat and its lead detoxification ability

Takafumi Mizuno, Masahiro Nakagawa, Hiroshi Ono, Daisuke Sugiura, Hideo Tamura, Hitoshi Obata. . . . . . . 39 [PDF]

A MRP homolog gene FeABCC1 was isolated from a lead-hyperaccumulating common buckwheat. The yeast transformed with FeABCC1 showed a marked increasing in lead tolerance, and accumulated more lead compared to control. Not similar to AtMRP3, FeABCC1 expression did not effect to cadmium tolerance in yeast.

A high-throughput Agrobacterium tumefaciens-mediated transformation system for molecular breeding and functional genomics of rice (Oryza sativa L.)

Ashok K. Shrawat, Allen G. Good. . . . . . . 47 [PDF]

Agrobacterium tumefaciens-mediated transformation is the preferred method for genetic transformation of rice. In this paper, we provided a comprehensive and high-through-put protocol for Agrobacterium-mediated transformation of rice and suggested its suitability for generating the large numbers of transgenic plants that are required for functional analysis and for evaluating traits of agronomic importance.

In vitro proliferation and triterpenoid characteristics of licorice (Glycyrrhiza uralensis Fischer, Leguminosae) stolons

Mareshige Kojoma, Kiyoshi Ohyama, Hikaru Seki, Yasuko Hiraoka, Sandra Naomi Asazu, Satoko Sawa, Haruo Sekizaki, Shigeo Yoshida, Toshiya Muranaka. . . . . . . 59 [PDF]

We describe the establishment of an in vitro stolon culture system for Glycyrrhiza uralensis. GC-MS analysis confirmed the accumulation of small amounts of glycyrrhizin in cultured stolons. Regenerated plants produced glycyrrhizin in their roots.

Reduced rice grain production under high-temperature stress closely correlates with ATP shortage during seed development

Kao-Chih She, Hiroaki Kusano, Mitsuhiro Yaeshima, Tadamasa Sasaki, Hikaru Satoh, Hiroaki Shimada. . . . . . . 67 [PDF]

Shortage of ATP content during seed development may occur by the high-temperature stress, which was closely correlated with insufficient seed starch production and deduced rice grain yield along with poor quality. Although rice cultivars Nipponbare and Taichung-65 showed typical symptoms in this case, a rice cultivar Kinmaze was suggested to possess some trait involved in tolerance to high-temperature stress.

Transformation of Arabidopsis by mutated acetolactate synthase genes from rice and Arabidopsis that confer specific resistance to pyrimidinylcarboxylate-type ALS inhibitors

Kiyoshi Kawai, Koichiro Kaku, Norihiko Izawa, Masanori Shimizu, Hirokazu Kobayashi, Tsutomu Shimizu. . . . . . . 75 [PDF]

We revealed that four mutated ALS genes derived from rice and Arabidopsis function as effective selectable markers for transformation of Arabidopsis. Moreover, the results of functional analysis of each transgenic Arabidopsis expressing the respective mutated ALS gene indicated that this selection system could be applied to a broad range of plant species.

Short Communication

Chloroplast biogenesis during the early stage of leaf development in rice

Kensuke Kusumi, Yoko Chono, Hiroshi Shimada, Eiji Gotoh, Michito Tsuyama, Koh Iba. . . . . . . 85 [PDF]

In rice, the developmental process in leaf formation can be divided into 7 stages (P0-P6). In this study, we investigated changes in the physiological and morphological state of chloroplasts in the developing leaves at the key stage P4, during which leaf blade structure established before emergence.

Isolation of Daucus carota ethylene insensitive3-like (DcEIL) involved in stress-inducible DcMYB1 expression in suspension-cultured carrot cells

Taira Miyahara, Shunsuke Satoh, Kazuhiro Maeda, Soichi Kimura, Nobuhiro Sasaki, Yoshihiro Ozeki. . . . . . . 91 [PDF]

In this work, we investigated a mechanism of stress-inducible DcMYB1 expression in phenylpropanoid metabolism. DcEIL cDNA was isolated from carrot suspension cultured-cells, which involved in suppression of DcMYB1 expression. DcEIL is considered to be regulated at posttranslational level in the ubiquitin-proteasome pathway.

Technical Note

Efficient induction of flower-color mutants by ion beam irradiation in petunia seedlings treated with high sucrose concentration

Yoshihiro Hase, Masachika Okamura, Daigaku Takeshita, Issay Narumi, Atsushi Tanaka. . . . . . . 99 [PDF]

We found that sucrose pretreatment significantly increased the frequency of flower-color mutants induced by ion beams in petunia. Since the frequency of chlorophyll mutants was not different between treated and non-treated group, our results suggest that sucrose pretreatment specifically increased the frequency of flower-color mutation.

Tissue Culture Note

Triterpenoid levels are reduced during Euphorbia tirucalli L. callus formation

Hidenobu Uchida, Kiyoshi Ohyama, Masashi Suzuki, Hirofumi Yamashita, Toshiya Muranaka, Kanji Ohyama. . . . . . . 105 [PDF]

In aerial parts of an oil plant Euphorbia tirucalli, 11 peaks of triterpenoid- and triterpenoid-like-peaks of total ion chromatogram (TIC) with a [M+] of 426, including those for euphol, β-amyrin, and glutinol, were detected. The ratio of these peaks to the total of all other peaks detected was ca. 86%. In the callus formation in this plant, the corresponding ratio decreased to only ca. 6.3%.

Transgenic Note

Cytoplasmic male sterility-associated ORF79 is toxic to plant regeneration when expressed with mitochondrial targeting sequence of ATPase γ subunit

Hisayo Kojima, Tomohiko Kazama, Sota Fujii, Kinya Toriyama. . . . . . . 111 [PDF] [Supplement 1] [Supplement 2]

To confirm the effect of ORF79, we introduced the orf79 gene with or without a mitochondrial targeting sequence into Taichung 65 carrying normal cytoplasm. It was revealed that ORF79 was toxic to plant regeneration when expressed as a fusion with mitochondrial targeting sequence of the ATPase γ subunit.

Gene Note

Characterization of the Arabidopsis Response Regulator Genes with Regard to Bisphenol A Signaling

Nobuyuki Terouchi, Chiaki Kato, Natsumi Hosoya. . . . . . . 115 [PDF]

Arabidopsis response regulator (ARR) are involved in the signal transduction mechanism induced by cytokinins. This study is the first to show that type-A ARR m-RNA accumulation is induced by bisphenol A. Bisphenol A is suggested to play some role in the mechanism.


Vol. 26 (No. 5). . . . . . . 119 [PDF]